Hotspot

rpoB-trnC-GCA

LSC · 27890-29013 · 1124 bp

Back to Ocotea

Pi 0.00297
MarkerSeek score 56.00
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1124
variable_sites
11
pi
0.00297
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 ATTCCCTCATTTCCATCCCG TTGTTAATCAGGCGACACCC 50.0 50.0 57.3 58.2 1230-1232 bp 1.0 73.9
rpoB-trnC-GCA_p2 ATTCCCTCATTTCCATCCCG CTGGGGAGAAAGGATTTGCA 50.0 50.0 57.3 57.8 1201-1203 bp 1.0 73.7
rpoB-trnC-GCA_p3 ATTCCCTCATTTCCATCCCG TGGGGAGAAAGGATTTGCAG 50.0 50.0 57.3 57.8 1200-1202 bp 1.0 73.7
rpoB-trnC-GCA_p4 ATTCCCTCATTTCCATCCCG AGAAAGGATTTGCAGTCCCC 50.0 50.0 57.3 57.8 1195-1197 bp 1.0 73.6
rpoB-trnC-GCA_p5 ATTCCCTCATTTCCATCCCG GGGGAGAAAGGATTTGCAGT 50.0 50.0 57.3 57.8 1199-1201 bp 1.0 73.6