Hotspot

atpH-atpI

LSC · 15055-15720 · 666 bp

Pi 0.00229

MarkerSeek score 57.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 4
pi: 0.00229
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.0	57.9	747-753 bp	1.0	80.5
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.9	57.9	739-745 bp	1.0	80.4
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.9	57.9	803-809 bp	1.0	79.8
atpH-atpI_p4	GATACCTTCTACGGCTTGGC	TTTTGCAACTTTAGCCGCAG	55.0	45.0	58.1	57.9	832-838 bp	1.0	79.6
atpH-atpI_p5	CGATACCTTCTACGGCTTGG	TTTTGCAACTTTAGCCGCAG	55.0	45.0	57.9	57.9	833-839 bp	1.0	79.6