Hotspot

trnK-UUU-rps16

LSC · 4387-5220 · 834 bp

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Pi 0.00362
MarkerSeek score 64.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
834
variable_sites
9
pi
0.00362
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 944-966 bp 1.0 81.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 943-965 bp 1.0 81.1
trnK-UUU-rps16_p3 AAAAGACCATAGCCGCACTT AAAAAGGCGCTCAACCTACA 45.0 45.0 57.8 58.0 965-987 bp 1.0 80.8
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 953-975 bp 1.0 80.1
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 947-969 bp 1.0 79.4