primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	103	505	456-470	1.000000	86.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	103	505	459-473	1.000000	85.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	103	505	470-484	1.000000	85.8
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	103	505	469-483	1.000000	85.8
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	103	505	471-485	1.000000	85.7
trnK-UUU_p1	trnK-UUU	1	TCGTATTTTCCGTACGATTGGT	GTCGGGCAATCGAATCTCTT	22	20	40.9	50.0	57.9	58.1	18.8	0.0	2.167374	1802	4386	2673-2679	1.000000	63.2
trnK-UUU_p2	trnK-UUU	2	TCGTATTTTCCGTACGATTGGT	AATGTTTTGACGAGGTCGGG	22	20	40.9	50.0	57.9	58.5	18.8	4.1	2.589290	1802	4386	2687-2693	1.000000	59.9
trnK-UUU_p3	trnK-UUU	3	CGTATTTTCCGTACGATTGGT	GTCGGGCAATCGAATCTCTT	21	20	42.9	50.0	56.5	58.1	18.8	0.0	2.601172	1802	4386	2672-2678	1.000000	59.8
trnK-UUU_p4	trnK-UUU	4	TCGTATTTTCCGTACGATTGGT	CAATGTTTTGACGAGGTCGG	22	20	40.9	50.0	57.9	57.4	18.8	4.1	2.703227	1802	4386	2688-2694	1.000000	58.9
trnK-UUU_p5	trnK-UUU	5	TCGTATTTTCCGTACGATTGGT	TCGGGCAATCGAATCTCTTT	22	20	40.9	45.0	57.9	57.3	18.8	0.0	2.800107	1802	4386	2672-2678	1.000000	58.2
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4387	5220	944-966	1.000000	81.1
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4387	5220	943-965	1.000000	81.1
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAAGACCATAGCCGCACTT	AAAAAGGCGCTCAACCTACA	20	20	45.0	45.0	57.8	58.0	0.0	7.6	0.224713	4387	5220	965-987	1.000000	80.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.3	58.0	5.3	7.6	0.307146	4387	5220	953-975	1.000000	80.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	20	20	50.0	50.0	58.2	58.2	20.3	7.6	0.397463	4387	5220	947-969	1.000000	79.4
rps16_p1	rps16	1	GGAGTCCCAACGAAAACCAT	AAGCAACGCATTTCTTCGTG	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.130919	5221	6329	1554-1565	1.000000	80.3
rps16_p2	rps16	2	GGGTCATGTGAGAGCAACAA	AAGCAACGCATTTCTTCGTG	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.140337	5221	6329	1424-1435	1.000000	80.3
rps16_p3	rps16	3	TGATACGGGGTCATGTGAGA	AAGCAACGCATTTCTTCGTG	20	20	50.0	45.0	57.8	58.0	0.0	0.0	0.189590	5221	6329	1431-1442	1.000000	79.9
rps16_p4	rps16	4	GGAGTCCCAACGAAAACCAT	AGTCACTTGCGATCTTTCCC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.194967	5221	6329	1729-1740	1.000000	79.7
rps16_p5	rps16	5	GGGTCATGTGAGAGCAACAA	AGTCACTTGCGATCTTTCCC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.204385	5221	6329	1599-1610	1.000000	79.7
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	15055	15720	747-753	1.000000	80.5
atpH-atpI_p2	atpH-atpI	2	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCCGCAG	20	20	45.0	45.0	57.9	57.9	1.4	0.0	0.195711	15055	15720	739-745	1.000000	80.4
atpH-atpI_p3	atpH-atpI	3	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCAG	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.245019	15055	15720	803-809	1.000000	79.8
atpH-atpI_p4	atpH-atpI	4	GATACCTTCTACGGCTTGGC	TTTTGCAACTTTAGCCGCAG	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.265574	15055	15720	832-838	1.000000	79.6
atpH-atpI_p5	atpH-atpI	5	CGATACCTTCTACGGCTTGG	TTTTGCAACTTTAGCCGCAG	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.268404	15055	15720	833-839	1.000000	79.6
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	ATTCCCTCATTTCCATCCCG	TTGTTAATCAGGCGACACCC	20	20	50.0	50.0	57.3	58.2	0.0	0.0	0.848327	27890	29013	1230-1232	1.000000	73.9
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	ATTCCCTCATTTCCATCCCG	CTGGGGAGAAAGGATTTGCA	20	20	50.0	50.0	57.3	57.8	0.0	0.0	0.885055	27890	29013	1201-1203	1.000000	73.7
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	ATTCCCTCATTTCCATCCCG	TGGGGAGAAAGGATTTGCAG	20	20	50.0	50.0	57.3	57.8	0.0	0.0	0.885055	27890	29013	1200-1202	1.000000	73.7
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	ATTCCCTCATTTCCATCCCG	AGAAAGGATTTGCAGTCCCC	20	20	50.0	50.0	57.3	57.8	0.0	14.6	0.886629	27890	29013	1195-1197	1.000000	73.6
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	ATTCCCTCATTTCCATCCCG	GGGGAGAAAGGATTTGCAGT	20	20	50.0	50.0	57.3	57.8	0.0	0.0	0.886629	27890	29013	1199-1201	1.000000	73.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CTTCACTAATCGGGGCCAAA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.9	58.1	4.4	0.0	0.230316	52725	54587	1999-2008	1.000000	79.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	ACTAATCGGGGCCAAAACTC	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.9	58.1	4.4	0.0	0.231809	52725	54587	1995-2004	1.000000	79.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CACTAATCGGGGCCAAAACT	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	58.2	58.1	4.4	0.0	0.273344	52725	54587	1996-2005	1.000000	78.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCACTAATCGGGGCCAAAAC	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	58.2	58.1	4.4	0.0	0.278553	52725	54587	1997-2006	1.000000	78.9
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTTCACTAATCGGGGCCAAA	ACGCATGTTGGGTCTTTGAA	20	20	50.0	45.0	57.9	58.3	4.4	0.0	0.436429	52725	54587	2062-2071	1.000000	77.6
petD_p1	petD	1	TCTCCGAAGAGAAGATGGAT	ACGCGTAGTATTTCACGGTT	20	20	45.0	45.0	55.0	57.6	0.0	11.7	3.302834	80254	81452	1248-1248	1.000000	54.0
petD_p2	petD	2	TCTCCGAAGAGAAGATGGATT	ACGCGTAGTATTTCACGGTT	21	20	42.9	45.0	55.9	57.6	0.0	11.7	3.475036	80254	81452	1248-1248	1.000000	52.6
petD_p3	petD	3	AGTTGAAGTACATTCTCCGAAGA	ACGCGTAGTATTTCACGGTT	23	20	39.1	45.0	57.3	57.6	0.0	11.7	4.076175	80254	81452	1261-1261	1.000000	47.8
petD_p4	petD	4	CTCCGAAGAGAAGATGGATT	ACGCGTAGTATTTCACGGTT	20	20	45.0	45.0	54.3	57.6	0.0	11.7	4.092295	80254	81452	1247-1247	1.000000	47.7
petD_p5	petD	5	TCTCCGAAGAGAAGATGGAT	TACGCGTAGTATTTCACGGT	20	20	45.0	45.0	55.0	56.8	0.0	16.4	4.153135	80254	81452	1249-1249	1.000000	47.2
rps19-rpl2_p1	rps19-rpl2	1	TGCCCGAGACCAAGTTACTA	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	58.1	58.0	0.0	22.4	0.096688	87902	87979	264-269	1.000000	81.0
rps19-rpl2_p2	rps19-rpl2	2	TGCCCGAGACCAAGTTACTA	CCTGCGCTTGGAAGAAAAAG	20	20	50.0	50.0	58.1	57.9	0.0	9.7	0.128607	87902	87979	259-264	1.000000	80.8
rps19-rpl2_p3	rps19-rpl2	3	TGCCCGAGACCAAGTTACTA	ATCCTGCGCTTGGAAGAAAA	20	20	50.0	45.0	58.1	58.1	0.0	22.4	0.162813	87902	87979	261-266	1.000000	80.5
rps19-rpl2_p4	rps19-rpl2	4	TGCCCGAGACCAAGTTACTA	CACAACCCCTTGGGGTTATC	20	20	50.0	55.0	58.1	58.2	0.0	35.0	0.225184	87902	87979	278-283	1.000000	79.9
rps19-rpl2_p5	rps19-rpl2	5	TGCCCGAGACCAAGTTACTA	GGCCCCGATTGGTAGAAAAA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.230461	87902	87979	302-307	1.000000	79.7
rpl2-rpl23_p1	rpl2-rpl23	1	CGGTATAGACGCTTATGGCC	ATACTGGAACTCGTGGGGAA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.060846	89478	89506	451-455	1.000000	80.8
rpl2-rpl23_p2	rpl2-rpl23	2	ATAGACGCTTATGGCCTCCT	ATACTGGAACTCGTGGGGAA	20	20	50.0	50.0	58.0	58.1	3.3	0.0	0.069920	89478	89506	447-451	1.000000	80.7
rpl2-rpl23_p3	rpl2-rpl23	3	GCTTATGGCCTCCTCCTCTA	ATACTGGAACTCGTGGGGAA	20	20	55.0	50.0	58.0	58.1	3.3	0.0	0.069920	89478	89506	441-445	1.000000	80.7
rpl2-rpl23_p4	rpl2-rpl23	4	TCTGTCCGTGCTTCAAACAA	ATACTGGAACTCGTGGGGAA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.084274	89478	89506	890-894	1.000000	80.1
rpl2-rpl23_p5	rpl2-rpl23	5	TTCTGTCCGTGCTTCAAACA	ATACTGGAACTCGTGGGGAA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.084274	89478	89506	891-895	1.000000	80.1
ycf2_p1	ycf2	1	CGGAACAAAGGGGGATACAG	AGATTCGTGGATCTCTCGGT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.098437	91995	92114	736-737	1.000000	87.1
ycf2_p2	ycf2	2	CGGAACAAAGGGGGATACAG	AACCTCGGACCAATCAATCG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.062562	91995	92114	887-888	1.000000	86.3
ycf2_p3	ycf2	3	CGGAACAAAGGGGGATACAG	TCGGCCCTATGAATGGAGAT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.068414	91995	92114	1030-1031	1.000000	85.6
ycf2_p4	ycf2	4	ATCCGGGATGGGATATGGTT	AACCTCGGACCAATCAATCG	20	20	50.0	50.0	57.9	58.0	9.2	0.0	0.083932	91995	92114	997-998	1.000000	85.3
ycf2_p5	ycf2	5	ATCCGGGATGGGATATGGTT	TCGGCCCTATGAATGGAGAT	20	20	50.0	50.0	57.9	58.0	9.2	0.0	0.089784	91995	92114	1140-1141	1.000000	84.8
ycf1_p1	ycf1	1	TGGTGGATGACTCAATCACT	TGAATCAAATCTTCTTTGTTCGGA	20	24	45.0	33.3	56.1	57.1	12.2	0.0	6.727152	114010	114129	251-251	1.000000	43.2
ycf1_p2	ycf1	2	TGGTGGATGACTCAATCACTTC	TGAATCAAATCTTCTTTGTTCGGA	22	24	45.5	33.3	58.1	57.1	12.2	0.0	6.933119	114010	114129	251-251	1.000000	43.2
ycf1_p3	ycf1	3	TGGTGGATGACTCAATCACT	CTGAATCAAATCTTCTTTGTTCGGA	20	25	45.0	36.0	56.1	58.1	12.2	11.9	6.948862	114010	114129	252-252	1.000000	43.2
ycf1_p4	ycf1	4	TGGTGGATGACTCAATCACT	TCTGAATCAAATCTTCTTTGTTCGG	20	25	45.0	36.0	56.1	58.1	12.2	11.4	6.948862	114010	114129	253-253	1.000000	43.2
ycf1_p5	ycf1	5	TGGTGGATGACTCAATCACTT	TGAATCAAATCTTCTTTGTTCGGA	21	24	42.9	33.3	56.9	57.1	12.2	0.0	6.951956	114010	114129	251-251	1.000000	43.2
ycf1-rps15_p1	ycf1-rps15	1	AATGGCAGCCGTTTCAGTAT	TGAAATTCAACAGACCCTTTGT	20	22	45.0	36.4	57.9	56.4	13.7	0.0	3.755555	118019	118339	441-460	1.000000	54.2
ycf1-rps15_p2	ycf1-rps15	2	ATAATGGCAGCCGTTTCAGT	TGAAATTCAACAGACCCTTTGT	20	22	45.0	36.4	57.9	56.4	24.3	0.0	3.755555	118019	118339	443-462	1.000000	54.2
ycf1-rps15_p3	ycf1-rps15	3	AATGGCAGCCGTTTCAGTAT	ACTTGAAATTCAACAGACCCT	20	21	45.0	38.1	57.9	55.3	13.7	4.0	3.813526	118019	118339	444-463	1.000000	53.7
ycf1-rps15_p4	ycf1-rps15	4	ATAATGGCAGCCGTTTCAGT	ACTTGAAATTCAACAGACCCT	20	21	45.0	38.1	57.9	55.3	24.3	4.0	3.813526	118019	118339	446-465	1.000000	53.7
ycf1-rps15_p5	ycf1-rps15	5	CCAATAATGGCAGCCGTTTC	TGAAATTCAACAGACCCTTTGT	20	22	50.0	36.4	57.8	56.4	24.3	0.0	3.835110	118019	118339	446-465	1.000000	53.5
ccsA-trnL-UAG_p1	ccsA-trnL-UAG	1	TGTTCTAAAGTGGCAAATATCA	TAGACACGCTGCTCTTAGGA	22	20	31.8	50.0	53.7	57.9	0.0	0.0	6.398430	127463	127526	148-154	1.000000	46.4
ccsA-trnL-UAG_p2	ccsA-trnL-UAG	2	TGTTCTAAAGTGGCAAATATCA	GGTAGACACGCTGCTCTTAG	22	20	31.8	55.0	53.7	57.8	0.0	0.0	6.470448	127463	127526	150-156	1.000000	46.4
ccsA-trnL-UAG_p3	ccsA-trnL-UAG	3	TGTTCTAAAGTGGCAAATATCA	GTAGACACGCTGCTCTTAGG	22	20	31.8	55.0	53.7	57.8	0.0	0.0	6.470448	127463	127526	149-155	1.000000	46.4
ccsA-trnL-UAG_p4	ccsA-trnL-UAG	4	TGTTCTAAAGTGGCAAATATCA	AAATTGGTAGACACGCTGCT	22	20	31.8	45.0	53.7	57.8	0.0	0.0	6.459157	127463	127526	155-161	1.000000	46.2
ccsA-trnL-UAG_p5	ccsA-trnL-UAG	5	TGTTCTAAAGTGGCAAATATCA	TTATTTCGATGAAGCCGCCA	22	20	31.8	45.0	53.7	58.0	0.0	0.0	6.305411	127463	127526	180-186	1.000000	45.3
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	TCCTAAGAGCAGCGTGTCTA	TTTTTGTGCGACAAACAAGT	20	20	50.0	35.0	57.9	55.2	0.0	12.1	2.967765	127607	129006	1451-1477	1.000000	61.0
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	AGCAGCGTGTCTACCAATTT	TTTTTGTGCGACAAACAAGT	20	20	45.0	35.0	57.8	55.2	0.0	12.1	3.028492	127607	129006	1444-1470	1.000000	60.6
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	CCTAAGAGCAGCGTGTCTAC	TTTTTGTGCGACAAACAAGT	20	20	55.0	35.0	57.8	55.2	0.0	12.1	3.039783	127607	129006	1450-1476	1.000000	60.4
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CTAAGAGCAGCGTGTCTACC	TTTTTGTGCGACAAACAAGT	20	20	55.0	35.0	57.8	55.2	0.0	12.1	3.039783	127607	129006	1449-1475	1.000000	60.4
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	GAGCAGCGTGTCTACCAATT	TTTTTGTGCGACAAACAAGT	20	20	50.0	35.0	58.3	55.2	0.0	12.1	3.116634	127607	129006	1445-1471	1.000000	59.8
rpl32-ndhF_p1	rpl32-ndhF	1	GTACGTTTCTTTGGAACCGC	GGAACAAGCAGAAGTCCCAA	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.323995	129181	130429	1335-1361	1.000000	81.5
rpl32-ndhF_p2	rpl32-ndhF	2	GTACGTTTCTTTGGAACCGC	AGCAGAAGTCCCAACCCTAT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.332664	129181	130429	1329-1355	1.000000	81.4
rpl32-ndhF_p3	rpl32-ndhF	3	GTACGTTTCTTTGGAACCGC	AAGCAGAAGTCCCAACCCTA	20	20	50.0	50.0	57.7	58.3	0.0	0.0	0.562944	129181	130429	1330-1356	1.000000	79.6
rpl32-ndhF_p4	rpl32-ndhF	4	GTACGTTTCTTTGGAACCGC	AACAAGCAGAAGTCCCAACC	20	20	50.0	50.0	57.7	58.3	0.0	0.0	0.612841	129181	130429	1333-1359	1.000000	79.2
rpl32-ndhF_p5	rpl32-ndhF	5	TACGTTTCTTTGGAACCGCC	GGAACAAGCAGAAGTCCCAA	20	20	50.0	50.0	58.8	58.0	0.0	0.0	0.790211	129181	130429	1334-1360	1.000000	77.7