Hotspot

ycf1

SSC · 122308-127842 · 5535 bp

Back to Ocimum

Pi 0.00882
MarkerSeek score 34.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5535
variable_sites
115
pi
0.00882
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCTCCTTTTTCATGTCCAAGCT ACGAAACCTTGGTTTATACC 40.9 40.0 57.9 53.2 214-220 bp 1.0 43.0
ycf1_p2 CGAGTTCTGCTTCTGTATTGC ACGAAACCTTGGTTTATACC 47.6 40.0 57.4 53.2 256-262 bp 1.0 42.5
ycf1_p3 CGAGTTCTGCTTCTGTATTGC AACGAAACCTTGGTTTATACC 47.6 38.1 57.4 54.1 257-263 bp 1.0 42.5
ycf1_p4 CGAGTTCTGCTTCTGTATTGC AAACGAAACCTTGGTTTATACC 47.6 36.4 57.4 54.9 258-264 bp 1.0 42.5
ycf1_p5 CGAGTTCTGCTTCTGTATTGC AAAACGAAACCTTGGTTTATACC 47.6 34.8 57.4 55.7 259-265 bp 1.0 42.5