Hotspot

rps15-ycf1

SSC · 121866-122307 · 442 bp

Back to Ocimum

Pi 0.0097
MarkerSeek score 36.90
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
442
variable_sites
10
pi
0.0097
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 CCTGAGTCAGTCTTCCATGC AACAGCAGTCGAGTTTCACA 55.0 45.0 58.0 57.7 1014-1022 bp 1.0 80.5
rps15-ycf1_p2 CCTGAGTCAGTCTTCCATGC TGTAGAACCGCTTCGTTTGT 55.0 45.0 58.0 57.8 1353 bp 0.75 68.0
rps15-ycf1_p3 AGTCGTTGACGTTTTCCCAA TGTAGAACCGCTTCGTTTGT 45.0 45.0 58.0 57.8 1162 bp 0.75 68.0
rps15-ycf1_p4 ATATTGCCCAGCTGTTCAGG TGTAGAACCGCTTCGTTTGT 50.0 45.0 57.9 57.8 1636 bp 0.75 67.7
rps15-ycf1_p5 CCAGCTGTTCAGGTCCATTT TGTAGAACCGCTTCGTTTGT 50.0 45.0 58.1 57.8 1629 bp 0.75 67.5