Hotspot

rpl32-trnL-UAG

SSC · 111985-112710 · 726 bp

Back to Ocimum

Pi 0.00836
MarkerSeek score 51.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
726
variable_sites
14
pi
0.00836
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGGCGTTAAAAGCGTTTTCT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.0 57.9 822-857 bp 1.0 83.4
rpl32-trnL-UAG_p2 CGGCGTTAAAAGCGTTTTCT AGCAGCGTGTCTACCAATTT 45.0 45.0 58.0 57.8 815-850 bp 1.0 83.0
rpl32-trnL-UAG_p3 CGGCGTTAAAAGCGTTTTCT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.0 57.8 821-856 bp 1.0 82.8
rpl32-trnL-UAG_p4 CGGCGTTAAAAGCGTTTTCT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.0 57.8 820-855 bp 1.0 82.8
rpl32-trnL-UAG_p5 CGGCGTTAAAAGCGTTTTCT GAGCAGCGTGTCTACCAATT 45.0 50.0 58.0 58.3 816-851 bp 1.0 82.3