Hotspot

psbT-pbf1

LSC · 73768-73832 · 65 bp

Back to Ocimum

Pi 0.033
MarkerSeek score 51.20
Primer available yes
Label psbT-pbf1

Feature Metadata

label_name
psbT-pbf1
feature_type
igs
region
LSC
length_bp
65
variable_sites
5
pi
0.033
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-pbf1_p1 TCGAGAACCGCCTAAAGTTC GGGTACGCCTTATATACCGC 50.0 55.0 57.6 57.7 174 bp 1.0 77.2
psbT-pbf1_p2 TCGAGAACCGCCTAAAGTTC GATCCATTCGAGGAACACGG 50.0 55.0 57.6 58.4 126 bp 1.0 76.9
psbT-pbf1_p3 TCGAGAACCGCCTAAAGTTC GTTTTACCGGGTACGCCTTA 50.0 50.0 57.6 57.6 182 bp 1.0 76.3
psbT-pbf1_p4 TCGAGAACCGCCTAAAGTTC TAAGTTTTACCGGGTACGCC 50.0 50.0 57.6 57.6 185 bp 1.0 76.3
psbT-pbf1_p5 GAGAACCGCCTAAAGTTCCA GGGTACGCCTTATATACCGC 50.0 55.0 57.5 57.7 172 bp 1.0 76.3