Hotspot

petA-psbJ

LSC · 62921-63966 · 1046 bp

Back to Ocimum

Pi 0.00464
MarkerSeek score 47.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1046
variable_sites
11
pi
0.00464
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TCCATTACGTGTCCAAGGTC TCCGGATTAGGTTCATCCCT 50.0 50.0 57.2 57.5 1150-1188 bp 1.0 73.4
petA-psbJ_p2 CCATTACGTGTCCAAGGTCT TCCGGATTAGGTTCATCCCT 50.0 50.0 57.2 57.5 1149-1187 bp 1.0 73.3
petA-psbJ_p3 TCCATTACGTGTCCAAGGTC CCGGATTAGGTTCATCCCTG 50.0 55.0 57.2 57.4 1149-1187 bp 1.0 72.9
petA-psbJ_p4 CCATTACGTGTCCAAGGTCT CCGGATTAGGTTCATCCCTG 50.0 55.0 57.2 57.4 1148-1186 bp 1.0 72.8
petA-psbJ_p5 TCGAGAAGGTTCAATTGGCT TCCGGATTAGGTTCATCCCT 45.0 50.0 57.1 57.5 1067-1105 bp 1.0 72.8