Hotspot

pafI-trnS-GGA

LSC · 44008-44843 · 836 bp

Back to Ocimum

Pi 0.00641
MarkerSeek score 49.10
Primer available yes
Label pafI-trnS-GGA

Feature Metadata

label_name
pafI-trnS-GGA
feature_type
igs
region
LSC
length_bp
836
variable_sites
13
pi
0.00641
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafI-trnS-GGA_p1 TGCCTCCTTTTCTCCTGAAG CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1008-1026 bp 1.0 73.3
pafI-trnS-GGA_p2 GCCTCCTTTTCTCCTGAAGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1007-1025 bp 1.0 73.3
pafI-trnS-GGA_p3 TGCCTCCTTTTCTCCTGAAG AAGTGAAGGTGCGGAAAGAG 50.0 50.0 57.1 58.1 1039-1057 bp 1.0 73.1
pafI-trnS-GGA_p4 GCCTCCTTTTCTCCTGAAGT AAGTGAAGGTGCGGAAAGAG 50.0 50.0 57.1 58.1 1038-1056 bp 1.0 73.1
pafI-trnS-GGA_p5 TGCCTCCTTTTCTCCTGAAG TTCCAATGCTACGCCTTGAA 50.0 45.0 57.1 57.8 979-997 bp 1.0 72.6