Hotspot

psaA-pafI

LSC · 41236-42038 · 803 bp

Pi 0.00943

MarkerSeek score 41.20

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 17
pi: 0.00943
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 41.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	896-922 bp	1.0	82.3
psaA-pafI_p2	GAAAAATGACCCGGTTTGGC	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.2	57.9	914-940 bp	1.0	81.4
psaA-pafI_p3	TTTGGCCCATTCCTCGAAAG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	900-926 bp	1.0	79.5
psaA-pafI_p4	TTTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	849-875 bp	1.0	79.5
psaA-pafI_p5	GGCCCATTCCTCGAAAGAAG	ATTGGTTGAAGATCACGGGG	55.0	50.0	58.6	57.9	897-923 bp	1.0	78.3