Hotspot

psbC-trnS-UGA

LSC · 34781-35018 · 238 bp

Back to Ocimum

Pi 0.0138
MarkerSeek score 31.20
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
238
variable_sites
7
pi
0.0138
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
31.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TTCTGTGGGTGGTGTAGCTA GAGAGATGGCTGAGTGGTTG 50.0 55.0 58.0 58.0 535-538 bp 1.0 81.9
psbC-trnS-UGA_p2 TTCTGTGGGTGGTGTAGCTA ATGGAGTCAGGGGAAATGGA 50.0 50.0 58.0 58.0 723-726 bp 1.0 81.8
psbC-trnS-UGA_p3 TTCTGTGGGTGGTGTAGCTA CATGGAGTCAGGGGAAATGG 50.0 55.0 58.0 57.9 724-727 bp 1.0 81.4
psbC-trnS-UGA_p4 ACCTACTGGACCAGAAGCTT ATGGAGTCAGGGGAAATGGA 50.0 50.0 58.0 58.0 1029-1032 bp 1.0 81.1
psbC-trnS-UGA_p5 ACCTACTGGACCAGAAGCTT GAGAGATGGCTGAGTGGTTG 50.0 55.0 58.0 58.0 841-844 bp 1.0 81.0