Hotspot

trnE-UUC-trnT-GGU

LSC · 30396-31015 · 620 bp

Back to Ocimum

Pi 0.00691
MarkerSeek score 47.40
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
620
variable_sites
10
pi
0.00691
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 750-761 bp 1.0 81.0
trnE-UUC-trnT-GGU_p2 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 729-740 bp 1.0 80.6
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 726-737 bp 1.0 80.4
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 712-723 bp 1.0 80.3
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC CCGATGACTTACGCCTTACC 55.0 55.0 57.8 58.2 747-758 bp 1.0 79.8