Hotspot

rpoB-trnC-GCA

LSC · 26815-28041 · 1227 bp

Back to Ocimum

Pi 0.00544
MarkerSeek score 46.30
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1227
variable_sites
16
pi
0.00544
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AGTTCTTCTGCCAAACCCTG TTTTGGTGATTCAGGCGACA 50.0 45.0 58.0 58.0 1367-1415 bp 1.0 83.3
rpoB-trnC-GCA_p2 GTTCTTCTGCCAAACCCTGA TTTTGGTGATTCAGGCGACA 50.0 45.0 58.0 58.0 1366-1414 bp 1.0 83.3
rpoB-trnC-GCA_p3 ATTGCCTCGTTTGTATCCCC TTTTGGTGATTCAGGCGACA 50.0 45.0 57.9 58.0 1289-1337 bp 1.0 83.2
rpoB-trnC-GCA_p4 AGTTCTTCTGCCAAACCCTG CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1346-1394 bp 1.0 82.9
rpoB-trnC-GCA_p5 GTTCTTCTGCCAAACCCTGA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1345-1393 bp 1.0 82.9