Hotspot

atpF-atpH

LSC · 12960-13264 · 305 bp

Back to Ocimum

Pi 0.00843
MarkerSeek score 44.40
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
305
variable_sites
6
pi
0.00843
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 CCAGTAGCCCAAAGAAACGA GGTTGGTTGTAGCACTAGCA 50.0 50.0 57.8 57.8 377-394 bp 1.0 81.9
atpF-atpH_p2 CCAGTAGCCCAAAGAAACGA GGGTTGGTTGTAGCACTAGC 50.0 55.0 57.8 58.5 378-395 bp 1.0 79.0
atpF-atpH_p3 CCAGTAGCCCAAAGAAACGA CCGAGGCAGAGGGAAAAATA 50.0 50.0 57.8 57.3 449-466 bp 1.0 76.9
atpF-atpH_p4 CCAGTAGCCCAAAGAAACGA TGGGTTGGTTGTAGCACTAG 50.0 50.0 57.8 57.1 379-396 bp 1.0 76.5
atpF-atpH_p5 CCAGTAGCCCAAAGAAACGA GCAGAGGGAAAAATACGAGGT 50.0 47.6 57.8 58.0 444-461 bp 1.0 74.6