Hotspot

trnR-UCU-atpA

LSC · 9982-10084 · 103 bp

Back to Ocimum

Pi 0.018
MarkerSeek score 45.30
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
103
variable_sites
6
pi
0.018
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AAAAATCGGAATGAACGGCG CGGACCGGTTTCTACTTCAG 45.0 55.0 57.7 58.0 224-225 bp 1.0 80.0
trnR-UCU-atpA_p2 AAAAATCGGAATGAACGGCG CCGGTTTCTACTTCAGGAGC 45.0 55.0 57.7 58.0 220-221 bp 1.0 80.0
trnR-UCU-atpA_p3 GAACGGCGTCCATTGTCTAA CCGGTTTCTACTTCAGGAGC 50.0 55.0 58.3 58.0 208-209 bp 1.0 80.0
trnR-UCU-atpA_p4 GAACGGCGTCCATTGTCTAA CGGACCGGTTTCTACTTCAG 50.0 55.0 58.3 58.0 212-213 bp 1.0 79.9
trnR-UCU-atpA_p5 ACGGCGTCCATTGTCTAATG CGGACCGGTTTCTACTTCAG 50.0 55.0 58.3 58.0 210-211 bp 1.0 79.4