Hotspot

rps16-trnQ-UUG

LSC · 5924-6836 · 913 bp

Back to Ocimum

Pi 0.00575
MarkerSeek score 38.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
913
variable_sites
12
pi
0.00575
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCCACAACGGGTCATATCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 581-1008 bp 1.0 83.4
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 559-986 bp 1.0 83.4
rps16-trnQ-UUG_p3 CCACAACGGGTCATATCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.3 58.1 580-1007 bp 1.0 78.8
rps16-trnQ-UUG_p4 TCCACAACGGGTCATATCCT TAGCAATTGAAATGGGGCGT 50.0 45.0 58.1 58.2 1068 bp 0.75 68.0
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TAGCAATTGAAATGGGGCGT 45.0 45.0 58.1 58.2 1046 bp 0.75 67.9