Hotspot

psbA-matK

LSC · 1447-2002 · 556 bp

Back to Ocimum

Pi 0.00642
MarkerSeek score 32.20
Primer available yes
Label psbA-matK

Feature Metadata

label_name
psbA-matK
feature_type
igs
region
LSC
length_bp
556
variable_sites
8
pi
0.00642
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
32.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-matK_p1 CGGAAACTAAGCTCCCACTC GGATTTTCGGGCGAACCTAT 55.0 50.0 58.0 58.0 1695-1711 bp 1.0 81.5
psbA-matK_p2 CGGAAACTAAGCTCCCACTC GAGGAACCCTGCATGCATTA 55.0 50.0 58.0 57.9 1667-1683 bp 1.0 81.3
psbA-matK_p3 GGAACCATGCATAGCACTGA GGATTTTCGGGCGAACCTAT 50.0 50.0 58.0 58.0 1939-1955 bp 1.0 81.0
psbA-matK_p4 CGGAAACTAAGCTCCCACTC AACCTATGGTTGGTCGAGGA 55.0 50.0 58.0 58.1 1698 bp 0.75 69.7
psbA-matK_p5 CGGAAACTAAGCTCCCACTC ACCTATGGTTGGTCGAGGAA 55.0 50.0 58.0 58.1 1697 bp 0.75 69.7