Hotspot

atpF

LSC · 12851-14108 · 1258 bp

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Pi 0
MarkerSeek score 26.10
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1258
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
26.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 CCATTGCCCCTCTTTCTTGT CTCGCTTTCTTCCTTCCCTC 50.0 55.0 58.1 58.0 2308-2317 bp 1.0 79.5
atpF_p2 CCATTGCCCCTCTTTCTTGT CGGGAAGGACTGATTTGAGG 50.0 55.0 58.1 58.0 2348-2357 bp 1.0 79.4
atpF_p3 CACTCACCGGTATTTGAGCA CTCGCTTTCTTCCTTCCCTC 50.0 55.0 57.9 58.0 1954-1963 bp 1.0 79.4
atpF_p4 CCATTGCCCCTCTTTCTTGT ATTTCTGCCGCTTCCGTTAT 50.0 45.0 58.1 58.0 2692-2701 bp 1.0 79.3
atpF_p5 CCATTGCCCCTCTTTCTTGT GCTGTAGAAGGTATCGCGAG 50.0 55.0 58.1 58.0 2602-2611 bp 1.0 79.3