Hotspot

atpB-rbcL

LSC · 57639-58421 · 783 bp

Back to Ochna

Pi 0.00904
MarkerSeek score 62.60
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
783
variable_sites
7
pi
0.00904
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 TTTCAAGCGTGGAAACTCCA GCCTTGAATCCAACACTTGC 45.0 50.0 58.0 57.9 868-876 bp 1.0 80.5
atpB-rbcL_p2 TTTCAAGCGTGGAAACTCCA CCAGCCTTGAATCCAACACT 45.0 50.0 58.0 58.1 871-879 bp 1.0 80.4
atpB-rbcL_p3 GATACGCCCCAGGTTCTTTT GCCTTGAATCCAACACTTGC 50.0 50.0 57.9 57.9 888-896 bp 1.0 79.9
atpB-rbcL_p4 GATACGCCCCAGGTTCTTTT CCAGCCTTGAATCCAACACT 50.0 50.0 57.9 58.1 891-899 bp 1.0 79.8
atpB-rbcL_p5 TGGAAACTCCAGGGTCAGAA GCCTTGAATCCAACACTTGC 50.0 50.0 58.2 57.9 859-867 bp 1.0 79.4