Hotspot

ndhC-trnV-UAC

LSC · 53493-54566 · 1074 bp

Back to Ochna

Pi 0.00373
MarkerSeek score 61.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1074
variable_sites
4
pi
0.00373
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGATAAAAGGCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1205 bp 1.0 78.1
ndhC-trnV-UAC_p2 CTAAGCGGGGATAAAAGGCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 1211 bp 1.0 75.6
ndhC-trnV-UAC_p3 GGGGATAAAAGGCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1204 bp 1.0 75.3
ndhC-trnV-UAC_p4 GGCCGGAAATTAGAAATGCC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.5 57.8 1195 bp 1.0 74.7
ndhC-trnV-UAC_p5 GGGGATAAAAGGCCGGAAAT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1193 bp 1.0 74.2