Hotspot

psbC-trnS-UGA

LSC · 38147-38380 · 234 bp

Back to Ochna

Pi 0.0342
MarkerSeek score 64.50
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
234
variable_sites
8
pi
0.0342
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TCATTTATGGCATGCGGGAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 57.9 58.1 411 bp 1.0 80.6
psbC-trnS-UGA_p2 TCATTTATGGCATGCGGGAA GATGGCCGAGTGGTTGATAG 45.0 55.0 57.9 58.1 422 bp 1.0 80.0
psbC-trnS-UGA_p3 TCATTTATGGCATGCGGGAA GGGTTCGAATCCCTCTCTCT 45.0 55.0 57.9 58.2 360 bp 1.0 79.5
psbC-trnS-UGA_p4 CATTTATGGCATGCGGGAAG GGTTGATAGCTCCGGTCTTG 50.0 55.0 57.6 58.1 410 bp 1.0 77.7
psbC-trnS-UGA_p5 TCATTTATGGCATGCGGGAA TGGTTGATAGCTCCGGTCTT 45.0 50.0 57.9 58.4 412 bp 1.0 77.6