Hotspot

petN-psbM

LSC · 29815-31123 · 1309 bp

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Pi 0.00387
MarkerSeek score 62.30
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1309
variable_sites
5
pi
0.00387
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTCTTCATTCT 50.0 42.9 58.2 57.0 1471-1490 bp 1.0 64.3
petN-psbM_p2 AGTATGGGGAAGAAGTGGGT TGCTACTGCACTCTTCATTCT 50.0 42.9 57.6 57.0 1413-1432 bp 1.0 62.7
petN-psbM_p3 TGGGGAAGAAGTGGGTTCTA TGCTACTGCACTCTTCATTCT 50.0 42.9 57.6 57.0 1409-1428 bp 1.0 62.1
petN-psbM_p4 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTCTTCATTC 50.0 45.0 58.2 55.5 1471-1490 bp 1.0 59.9
petN-psbM_p5 ATAGTAAGTCTCGCTTGGGC TGCTACTGCACTCTTCATTCT 50.0 42.9 57.1 57.0 1472-1491 bp 1.0 58.3