Hotspot

trnK-UUU-rps16

LSC · 4376-5298 · 923 bp

Back to Ochna

Pi 0.00881
MarkerSeek score 57.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
923
variable_sites
8
pi
0.00881
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 ATGGACGAAGCCCTTTCTTC TGAGTTGGTTTTCGCTGGAA 50.0 45.0 57.9 58.0 1084-1098 bp 1.0 80.4
trnK-UUU-rps16_p2 GGACGAAGCCCTTTCTTCAT TGAGTTGGTTTTCGCTGGAA 50.0 45.0 57.9 58.0 1082-1096 bp 1.0 80.4
trnK-UUU-rps16_p3 ATGGACGAAGCCCTTTCTTC AGTTGGTTTTCGCTGGAACT 50.0 45.0 57.9 57.9 1082-1096 bp 1.0 80.3
trnK-UUU-rps16_p4 GGACGAAGCCCTTTCTTCAT AGTTGGTTTTCGCTGGAACT 50.0 45.0 57.9 57.9 1080-1094 bp 1.0 80.3
trnK-UUU-rps16_p5 GGACGAAGCCCTTTCTTCAT ATGAGTTGGTTTTCGCTGGA 50.0 45.0 57.9 57.7 1083-1097 bp 1.0 78.6