Hotspot

trnH-GUG-psbA

LSC · 85-463 · 379 bp

Back to Ochna

Pi 0.0185
MarkerSeek score 64.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
379
variable_sites
7
pi
0.0185
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG AGCTGCTGTTGAAGTTCCAT 55.0 45.0 58.1 57.7 435-437 bp 1.0 78.0
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 449-451 bp 1.0 77.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 448-450 bp 1.0 77.9
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 450-452 bp 1.0 77.9
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 55.0 55.0 58.1 58.3 498-500 bp 1.0 77.4