Hotspot

ndhE

SSC · 121905-122210 · 306 bp

Back to Ochagavia

Pi 0.00654

MarkerSeek score 41.50

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 2
pi: 0.00654
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GTAGACACCAGACGAAGCAG	GGGTCACTTCGATGGTTTGT	55.0	50.0	58.0	58.1	1014-1041 bp	1.0	80.5
ndhE_p2	ATTTGACCAGAAAAGCCCGT	GGGTCACTTCGATGGTTTGT	45.0	50.0	58.0	58.1	1485-1512 bp	1.0	80.3
ndhE_p3	GTAGACACCAGACGAAGCAG	TTGTATTAGGGGGTCTGGGG	55.0	55.0	58.0	58.1	1251-1278 bp	1.0	80.3
ndhE_p4	ATTTGACCAGAAAAGCCCGT	TTGTATTAGGGGGTCTGGGG	45.0	55.0	58.0	58.1	1722-1749 bp	1.0	80.2
ndhE_p5	CATGCTGCGAGTTGTTTCAG	TTGTATTAGGGGGTCTGGGG	50.0	55.0	58.0	58.1	1601-1628 bp	1.0	79.9