Hotspot

psaC-ndhE

SSC · 121518-121904 · 387 bp

Back to Ochagavia

Pi 0.0284

MarkerSeek score 65.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 387
variable_sites: 11
pi: 0.0284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TTGCTTGGCTTTACATCCGT	TTATAGCCATTGCAGCTGCT	45.0	45.0	58.1	57.6	611-630 bp	1.0	77.8
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	TTATAGCCATTGCAGCTGCT	50.0	45.0	58.1	57.6	599-618 bp	1.0	77.6
psaC-ndhE_p3	TTGCTTGGCTTTACATCCGT	TATAGCCATTGCAGCTGCTG	45.0	50.0	58.1	58.4	610-629 bp	1.0	77.5
psaC-ndhE_p4	CCAATACATCTGTGGGGCAA	TTATAGCCATTGCAGCTGCT	50.0	45.0	57.9	57.6	576-595 bp	1.0	77.5
psaC-ndhE_p5	TTGCTTGGCTTTACATCCGT	GTTATAGCCATTGCAGCTGC	45.0	50.0	58.1	57.6	612-631 bp	1.0	77.4