Hotspot

ndhF-rpl32

SSC · 116351-117467 · 1117 bp

Back to Ochagavia

Pi 0.0204
MarkerSeek score 56.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1117
variable_sites
14
pi
0.0204
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.61
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGAAAAGGTATTATCCACGCA GGTGGAAACCGATTTCGCTA 38.1 50.0 55.0 58.3 847-1278 bp 1.0 51.0
ndhF-rpl32_p2 AAGAAAAGGTATTATCCACGCA GGTGGAAACCGATTTCGCTA 36.4 50.0 55.8 58.3 848-1279 bp 1.0 49.3
ndhF-rpl32_p3 AGAAAAGGTATTATCCACGCAT GGTGGAAACCGATTTCGCTA 36.4 50.0 55.6 58.3 847-1278 bp 1.0 47.5
ndhF-rpl32_p4 GAAAGAAAAGGTATTATCCACGCA GGTGGAAACCGATTTCGCTA 37.5 50.0 57.6 58.3 850-1281 bp 1.0 47.4
ndhF-rpl32_p5 TGAAAGAAAAGGTATTATCCACGC GGTGGAAACCGATTTCGCTA 37.5 50.0 57.6 58.3 851-1282 bp 1.0 47.4