Hotspot

rps8-rpl14

IRb · 83983-84136 · 154 bp

Back to Ochagavia

Pi 0.0195

MarkerSeek score 63.70

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: IRb
length_bp: 154
variable_sites: 3
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGATCGGCAATAGTGTCCCT	GAACTCGAGTTTTTGGTGCG	50.0	50.0	58.2	58.0	266-276 bp	1.0	79.1
rps8-rpl14_p2	AGATCGGCAATAGTGTCCCT	ACTCGAGTTTTTGGTGCGAT	50.0	45.0	58.2	58.1	264-274 bp	1.0	78.3
rps8-rpl14_p3	AGATCGGCAATAGTGTCCCT	AACTCGAGTTTTTGGTGCGA	50.0	45.0	58.2	58.3	265-275 bp	1.0	76.5
rps8-rpl14_p4	AGATCGGCAATAGTGTCCCT	GGAACTCGAGTTTTTGGTGC	50.0	50.0	58.2	57.6	267-277 bp	1.0	76.0
rps8-rpl14_p5	AGATCGGCAATAGTGTCCCT	CTCGAGTTTTTGGTGCGATC	50.0	50.0	58.2	57.5	263-273 bp	1.0	75.2