Hotspot

psbE-petL

LSC · 67602-69089 · 1488 bp

Back to Ochagavia

Pi 0.0135

MarkerSeek score 57.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1488
variable_sites: 20
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCAGCCAGTATAAAACCGA	50.0	45.0	57.9	56.9	1634-1654 bp	1.0	72.1
psbE-petL_p2	ACTGACGAATAACCAACCCG	AGCAGCCAGTATAAAACCGA	50.0	45.0	57.9	56.9	1647-1667 bp	1.0	71.8
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCAGCCAGTATAAAACCGA	45.0	45.0	58.2	56.9	1643-1663 bp	1.0	71.2
psbE-petL_p4	CGAATAACCAACCCGCAATG	AGCAGCCAGTATAAAACCGA	50.0	45.0	57.8	56.9	1642-1662 bp	1.0	71.0
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGCAGCCAGTATAAAACCGA	50.0	45.0	58.2	56.9	1636-1656 bp	1.0	70.6