Hotspot

petA-psbJ

LSC · 65740-66832 · 1093 bp

Back to Ochagavia

Pi 0.00642

MarkerSeek score 62.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1093
variable_sites: 7
pi: 0.00642
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCTGGGTTGGGTTCATCTCT	50.0	50.0	57.9	58.0	1219-1236 bp	1.0	79.8
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	TGGGTTGGGTTCATCTCTCT	50.0	50.0	57.9	58.0	1217-1234 bp	1.0	79.8
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	CTGGGTTGGGTTCATCTCTC	50.0	55.0	57.9	57.6	1218-1235 bp	1.0	76.9
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	TCTGGGTTGGGTTCATCTCT	50.0	50.0	58.7	58.0	1226-1243 bp	1.0	74.8
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	TGGGTTGGGTTCATCTCTCT	50.0	50.0	58.7	58.0	1224-1241 bp	1.0	74.8