Hotspot

rpoC1-rpoB

LSC · 24913-24938 · 26 bp

Back to Ochagavia

Pi 0.0385

MarkerSeek score 74.60

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 1
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	GGTAGGATTTTGTTGGCCCA	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.1	58.0	205 bp	1.0	79.5
rpoC1-rpoB_p2	AGGTAGGATTTTGTTGGCCC	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.8	58.0	206 bp	1.0	78.2
rpoC1-rpoB_p3	GGGTTTTGTCACCTCTCCAA	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.6	58.0	239 bp	1.0	77.1
rpoC1-rpoB_p4	GGTAGGATTTTGTTGGCCCA	TTCGATTGCTCGTTCGAGAA	50.0	45.0	58.1	57.7	210 bp	1.0	77.1
rpoC1-rpoB_p5	GGTAGGATTTTGTTGGCCCA	TTTCGATTGCTCGTTCGAGA	50.0	45.0	58.1	57.7	211 bp	1.0	77.1