Hotspot

ndhB

IRb · 95678-97423 · 1746 bp

Back to Oberonia

Pi 0.01
MarkerSeek score 47.20
Primer available yes
Label ndhB

Feature Metadata

label_name
ndhB
feature_type
gene
region
IRb
length_bp
1746
variable_sites
14
pi
0.01
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.71
markerseek_score
47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB_p1 TCGAATGTGATAAGTTCCGGT TCGTAGATTGGATGCAGTTACT 42.9 40.9 57.1 57.0 1857 bp 0.333333 14.3
ndhB_p2 TCTCGAATGTGATAAGTTCCGG TCGTAGATTGGATGCAGTTACT 45.5 40.9 58.0 57.0 1859 bp 0.333333 13.3
ndhB_p3 TCGAATGTGATAAGTTCCGGT TTCGTAGATTGGATGCAGTT 42.9 40.0 57.1 54.8 1858 bp 0.333333 13.3
ndhB_p4 TCGAATGTGATAAGTTCCGGT TTTCGTAGATTGGATGCAGT 42.9 40.0 57.1 54.8 1859 bp 0.333333 13.3
ndhB_p5 TCGAATGTGATAAGTTCCGGT TTCGTAGATTGGATGCAGTTACT 42.9 39.1 57.1 57.7 1858 bp 0.333333 13.3