Hotspot

ccsA-psaC

SSC · 112421-112993 · 573 bp

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Pi 0.0445

MarkerSeek score 76.10

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: SSC
length_bp: 573
variable_sites: 37
pi: 0.0445
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	ATGCAGCTCTTTTATGCGGA	CGGATTTCTTGAGTGTCCGT	45.0	50.0	58.0	57.9	1158-1168 bp	1.0	84.0
ccsA-psaC_p2	TGCAGCTCTTTTATGCGGAT	CGGATTTCTTGAGTGTCCGT	45.0	50.0	58.0	57.9	1157-1167 bp	1.0	84.0
ccsA-psaC_p3	TGCAGCTCTTTTATGCGGAT	GTACCCAATGTGTTCGAGCT	45.0	50.0	58.0	57.9	1295-1305 bp	1.0	83.5
ccsA-psaC_p4	ATGCAGCTCTTTTATGCGGA	GTACCCAATGTGTTCGAGCT	45.0	50.0	58.0	57.9	1296-1306 bp	1.0	83.4
ccsA-psaC_p5	TTTTACCCAAGGTTTCGCCA	CGGATTTCTTGAGTGTCCGT	45.0	50.0	57.9	57.9	1277-1287 bp	0.666667	68.2