Hotspot

trnN-GUU-rpl32

SSC · 109260-110522 · 1263 bp

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Pi 0.102
MarkerSeek score 89.00
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
SSC
length_bp
1263
variable_sites
175
pi
0.102
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
89.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 AGTCAGTTAACAGCCAACCG TGACATAGAAGTGCGTTTTT 50.0 35.0 58.1 53.5 1339-1477 bp 1.0 54.7
trnN-GUU-rpl32_p2 AGTCAGTTAACAGCCAACCG TTGACATAGAAGTGCGTTTT 50.0 35.0 58.1 53.5 1340-1478 bp 1.0 54.7
trnN-GUU-rpl32_p3 AGTCAGTTAACAGCCAACCG TTTGACATAGAAGTGCGTTT 50.0 35.0 58.1 53.5 1341-1479 bp 1.0 54.7
trnN-GUU-rpl32_p4 AGTCAGTTAACAGCCAACCG TTTTGACATAGAAGTGCGTT 50.0 35.0 58.1 53.5 1342-1480 bp 1.0 54.7
trnN-GUU-rpl32_p5 AGTCAGTTAACAGCCAACCG TTTTTGACATAGAAGTGCGT 50.0 35.0 58.1 53.5 1343-1481 bp 1.0 54.7