Hotspot

psbB-psbT

LSC · 73523-74116 · 594 bp

Back to Oberonia

Pi 0.0484

MarkerSeek score 77.80

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 594
variable_sites: 36
pi: 0.0484
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TGGAGATCCAACTACAAGGAGA	GGAATGTATAAACCAATGCTTCCA	45.5	37.5	57.9	57.5	447-668 bp	1.0	48.9
psbB-psbT_p2	TGGAGATCCAACTACAAGGAGA	AGGAATGTATAAACCAATGCTTCC	45.5	37.5	57.9	57.3	448-669 bp	1.0	48.9
psbB-psbT_p3	GGAGATCCAACTACAAGGAGAC	GGAATGTATAAACCAATGCTTCCA	50.0	37.5	57.6	57.5	446-667 bp	1.0	48.9
psbB-psbT_p4	TGGAGATCCAACTACAAGGAGA	AGTCGAAACTAAGAGGAATGT	45.5	38.1	57.9	54.3	461-682 bp	1.0	48.8
psbB-psbT_p5	TGGAGATCCAACTACAAGGAGA	AAGTCGAAACTAAGAGGAATGT	45.5	36.4	57.9	55.1	462-683 bp	1.0	48.8