Hotspot

psbE-petL

LSC · 63758-64994 · 1237 bp

Back to Oberonia

Pi 0.0402

MarkerSeek score 78.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1237
variable_sites: 71
pi: 0.0402
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAAAAGAACGTTCTCCCGTG	GCTGGGGTTATAGTTGAAGCA	50.0	47.6	57.3	58.0	1314-1327 bp	1.0	75.0
psbE-petL_p2	CAAAAGAACGTTCTCCCGTG	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.3	58.0	1310-1323 bp	1.0	75.0
psbE-petL_p3	CAAAAGAACGTTCTCCCGTG	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.3	58.0	1312-1325 bp	1.0	75.0
psbE-petL_p4	CAGCAAAAGAACGTTCTCCC	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.3	58.0	1313-1326 bp	1.0	74.8
psbE-petL_p5	CAGCAAAAGAACGTTCTCCC	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.3	58.0	1315-1328 bp	1.0	74.8