Hotspot

petA-psbJ

LSC · 61936-62993 · 1058 bp

Back to Oberonia

Pi 0.0414

MarkerSeek score 76.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1058
variable_sites: 62
pi: 0.0414
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	CGGGTTGGGTTCATCTCTTT	50.0	50.0	58.0	57.8	1085-1166 bp	1.0	86.7
petA-psbJ_p2	TGGCATCTGTGATTTTGGCA	CGGGTTGGGTTCATCTCTTT	45.0	50.0	58.4	57.8	1086-1167 bp	1.0	83.8
petA-psbJ_p3	GGCATCTGTGATTTTGGCAC	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.0	57.3	1090-1171 bp	1.0	82.6
petA-psbJ_p4	GGCATCTGTGATTTTGGCAC	CTATTCCGGGTTGGGTTCAT	50.0	50.0	58.0	57.3	1091-1172 bp	1.0	82.5
petA-psbJ_p5	GCATCTGTGATTTTGGCACA	CGGGTTGGGTTCATCTCTTT	45.0	50.0	57.3	57.8	1084-1165 bp	1.0	80.9