Hotspot

trnE-UUC-trnT-GGU

LSC · 30857-32179 · 1323 bp

Back to Oberonia

Pi 0.0601
MarkerSeek score 79.80
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1323
variable_sites
96
pi
0.0601
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 820-1427 bp 1.0 88.9
trnE-UUC-trnT-GGU_p2 CGTTGCCTCCTTGAAAGAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.8 58.1 799-1406 bp 1.0 88.6
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA TGGCGTTACTCTACCACTGA 50.0 50.0 57.8 58.1 796-1403 bp 1.0 88.4
trnE-UUC-trnT-GGU_p4 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 798-1405 bp 1.0 88.1
trnE-UUC-trnT-GGU_p5 CGTTGCCTCCTTGAAAGAGA CCGATGACTTACGCCTTACC 50.0 55.0 57.8 58.2 817-1424 bp 1.0 87.8