Hotspot

psbM-trnD-GUC

LSC · 29339-30307 · 969 bp

Back to Oberonia

Pi 0.0415

MarkerSeek score 76.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 969
variable_sites: 58
pi: 0.0415
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CAGCGCAGTAGCAATAAACG	TTCGTCGCTCCATGAAGAAG	50.0	50.0	57.9	58.0	1306-1337 bp	0.666667	70.4
psbM-trnD-GUC_p2	CAGCGCAGTAGCAATAAACG	CCATGCATATGGCTGGGATT	50.0	50.0	57.9	58.1	1073-1096 bp	0.666667	69.9
psbM-trnD-GUC_p3	ATGAATCTTTTCGAGGGGCC	TTCGTCGCTCCATGAAGAAG	50.0	50.0	57.9	58.0	1851 bp	0.333333	52.8
psbM-trnD-GUC_p4	ATGAATCTTTTCGAGGGGCC	CCATGCATATGGCTGGGATT	50.0	50.0	57.9	58.1	1610 bp	0.333333	52.3
psbM-trnD-GUC_p5	ATGAATCTTTTCGAGGGGCC	AGTTCAATCGGTCAGAGCAC	50.0	50.0	57.9	57.9	1588 bp	0.333333	52.2