Hotspot

petN-psbM

LSC · 28446-29233 · 788 bp

Back to Oberonia

Pi 0.0429

MarkerSeek score 78.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 46
pi: 0.0429
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.2	58.3	848-936 bp	1.0	84.9
petN-psbM_p2	TAGTATGGGGAAGGAGTGGG	ACGTCAAAACAGTCAGCCAA	55.0	45.0	57.5	58.3	791-879 bp	1.0	82.4
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TACGTCAAAACAGTCAGCCA	50.0	45.0	58.2	57.4	849-937 bp	1.0	82.1
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	CGTCAAAACAGTCAGCCAAA	50.0	45.0	58.2	57.2	847-935 bp	1.0	80.2
petN-psbM_p5	TAGTATGGGGAAGGAGTGGG	TACGTCAAAACAGTCAGCCA	55.0	45.0	57.5	57.4	792-880 bp	1.0	79.6