Hotspot

trnK-UUU-rps16

LSC · 4297-4731 · 435 bp

Back to Oberonia

Pi 0.045

MarkerSeek score 77.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 435
variable_sites: 29
pi: 0.045
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TACCGTTGAGTTAGCAACCC	AGAGAATTTCAAGTTTCTTTGGA	50.0	30.4	57.5	54.4	491-538 bp	1.0	47.9
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	AGAGAATTTCAAGTTTCTTTGGA	50.0	30.4	58.2	54.4	505-552 bp	1.0	47.7
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	AGAGAATTTCAAGTTTCTTTGGA	50.0	30.4	58.2	54.4	504-551 bp	1.0	47.7
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	AGAGAATTTCAAGTTTCTTTGGAT	50.0	29.2	58.2	54.9	504-551 bp	1.0	47.7
trnK-UUU-rps16_p5	AGGATCAGTCGCGGTCTTAT	AGAGAATTTCAAGTTTCTTTGGA	50.0	30.4	58.3	54.4	590-637 bp	1.0	46.6