Hotspot

ycf3

LSC · 43690-45652 · 1963 bp

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Pi 0
MarkerSeek score 52.50
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
1963
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 ACTGATGGGACCAACAAACA GGATCATTTTCCCACGAGAAG 45.0 47.6 57.3 56.7 2101-2105 bp 1.0 55.6
ycf3_p2 ACTGATGGGACCAACAAACA GGATCATTTTCCCACGAGAAGT 45.0 45.5 57.3 58.4 2101-2105 bp 1.0 55.1
ycf3_p3 ACTGATGGGACCAACAAACA GGATCATTTTCCCACGAGAA 45.0 45.0 57.3 55.4 2101-2105 bp 1.0 53.7
ycf3_p4 ACTGATGGGACCAACAAACAA GGATCATTTTCCCACGAGAAG 42.9 47.6 58.0 56.7 2101-2105 bp 1.0 53.4
ycf3_p5 TGACTGATGGGACCAACAAA GGATCATTTTCCCACGAGAAG 45.0 47.6 57.0 56.7 2103-2107 bp 1.0 53.3