Hotspot

psbD

LSC · 34025-35086 · 1062 bp

Pi 0.00126

MarkerSeek score 47.10

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.00126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	TGAGGTGTTCGGAAATGGTC	GTTTCTTGATCACGACCGGA	50.0	50.0	57.8	57.9	1143 bp	1.0	78.1
psbD_p2	TGATCGAATCTTCAGACGCC	GTTTCTTGATCACGACCGGA	50.0	50.0	57.8	57.9	1175 bp	1.0	77.9
psbD_p3	TGAGGTGTTCGGAAATGGTC	ACCGGTGGTTTCTTGATCAC	50.0	50.0	57.8	58.1	1150 bp	1.0	77.8
psbD_p4	GCAGTGCGCAAGAAATCAAA	GTTTCTTGATCACGACCGGA	45.0	50.0	58.2	57.9	1206 bp	1.0	77.7
psbD_p5	TGATCGAATCTTCAGACGCC	ACCGGTGGTTTCTTGATCAC	50.0	50.0	57.8	58.1	1182 bp	1.0	77.6