Hotspot

ndhI

SSC · 121176-121712 · 537 bp

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Pi 0.00248
MarkerSeek score 36.50
Primer available yes
Label ndhI

Feature Metadata

label_name
ndhI
feature_type
gene
region
SSC
length_bp
537
variable_sites
2
pi
0.00248
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI_p1 CGGTTCTCACCAACCAATCA TCACGACTTGTCTCAAACAA 50.0 40.0 58.1 55.2 682-683 bp 1.0 56.9
ndhI_p2 CGGTTCTCACCAACCAATCA TTCACGACTTGTCTCAAACA 50.0 40.0 58.1 55.2 683-684 bp 1.0 56.9
ndhI_p3 CAAATTAAGCCGCGGTTCTC TCACGACTTGTCTCAAACAA 50.0 40.0 57.8 55.2 694-695 bp 1.0 56.4
ndhI_p4 CAAATTAAGCCGCGGTTCTC TTCACGACTTGTCTCAAACA 50.0 40.0 57.8 55.2 695-696 bp 1.0 56.4
ndhI_p5 AAATTAAGCCGCGGTTCTCA TCACGACTTGTCTCAAACAA 45.0 40.0 58.2 55.2 693-694 bp 1.0 56.3