Hotspot

ndhB

IRa · 143999-146210 · 2212 bp

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Pi 0.00151
MarkerSeek score 45.70
Primer available yes
Label ndhB

Feature Metadata

label_name
ndhB
feature_type
gene
region
IRa
length_bp
2212
variable_sites
5
pi
0.00151
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB_p1 TCCTATTCATGGAGATTCCGT ATAACCCTAGCCCATTCCCA 42.9 50.0 55.9 57.8 2406 bp 1.0 53.8
ndhB_p2 TCCTATTCATGGAGATTCCGT TAACCCTAGCCCATTCCCAT 42.9 50.0 55.9 57.8 2405 bp 1.0 53.8
ndhB_p3 TCCTATTCATGGAGATTCCGT TTTGGGCGGAACAGATCTAC 42.9 50.0 55.9 57.6 2385 bp 1.0 52.4
ndhB_p4 TCCTATTCATGGAGATTCCGT TTGGGCGGAACAGATCTACT 42.9 50.0 55.9 58.4 2384 bp 1.0 52.2
ndhB_p5 TCCTATTCATGGAGATTCCGT TGGGCGGAACAGATCTACTA 42.9 50.0 55.9 57.6 2383 bp 1.0 52.0