Hotspot

ycf4-cemA

LSC · 61757-62671 · 915 bp

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Pi 0.00146
MarkerSeek score 57.70
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
915
variable_sites
2
pi
0.00146
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TTGGCCTATTTCTTGCGTGT GAGAAATCCACCAGGGCAAA 45.0 50.0 58.1 58.1 1027 bp 1.0 78.8
ycf4-cemA_p2 ACAAAAAGCTGCCGAATTGG GAGAAATCCACCAGGGCAAA 45.0 50.0 57.8 58.1 1043 bp 1.0 78.3
ycf4-cemA_p3 TTGGCCTATTTCTTGCGTGT AAAGAGAAATCCACCAGGGC 45.0 50.0 58.1 57.8 1030 bp 1.0 77.6
ycf4-cemA_p4 ACAAAAAGCTGCCGAATTGG AAAGAGAAATCCACCAGGGC 45.0 50.0 57.8 57.8 1046 bp 1.0 77.1
ycf4-cemA_p5 TTGGCCTATTTCTTGCGTGT AGAAATCCACCAGGGCAAAA 45.0 45.0 58.1 57.6 1026 bp 1.0 76.2