Hotspot

petN-psbM

LSC · 29453-30578 · 1126 bp

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Pi 0.00178
MarkerSeek score 59.90
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1126
variable_sites
3
pi
0.00178
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1288-1289 bp 1.0 67.7
petN-psbM_p2 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 1227-1228 bp 1.0 66.1
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTC 50.0 45.0 58.2 56.1 1288-1289 bp 1.0 63.5
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTC 50.0 45.0 57.6 56.1 1227-1228 bp 1.0 61.9
petN-psbM_p5 ATAGTAAGTCTCGCTTGGGC TGCTACTGCACTGTTCATTCT 50.0 42.9 57.1 57.6 1289-1290 bp 1.0 61.8