Hotspot

trnS-GCU-trnG-UCC

LSC · 8694-9500 · 807 bp

Back to Nyssa

Pi 0.00167
MarkerSeek score 55.10
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
807
variable_sites
2
pi
0.00167
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGAATCAAACCAAAAGACCCCT 50.0 40.9 58.0 58.1 912-921 bp 1.0 64.3
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGAATCAAACCAAAAGACCCCT 50.0 40.9 58.0 58.1 911-920 bp 1.0 64.3
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA TGAATCAAACCAAAAGACCCCT 50.0 40.9 58.2 58.1 966-975 bp 1.0 63.1
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA TGAATCAAACCAAAAGACCCC 50.0 42.9 58.0 56.6 912-921 bp 1.0 61.8
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC TGAATCAAACCAAAAGACCCC 50.0 42.9 58.0 56.6 911-920 bp 1.0 61.8