Hotspot

ccsA-ndhD

SSC · 114290-114559 · 270 bp

Back to Nymphoides

Pi 0.0427

MarkerSeek score 72.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 270
variable_sites: 26
pi: 0.0427
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AAATTCCGCAATTGTGGCAG	CGGAGTAGCTATCCCAATGC	45.0	55.0	57.9	57.9	765-791 bp	1.0	85.6
ccsA-ndhD_p2	AAATTCCGCAATTGTGGCAG	GCGGAGTAGCTATCCCAATG	45.0	55.0	57.9	57.9	766-792 bp	1.0	85.6
ccsA-ndhD_p3	AAATTCCGCAATTGTGGCAG	CTCCCGGCCAACGTAATTTA	45.0	50.0	57.9	58.0	976-1002 bp	1.0	84.6
ccsA-ndhD_p4	ACCCAAGGCTTTGCTACTTC	CGGAGTAGCTATCCCAATGC	50.0	55.0	58.1	57.9	1280-1306 bp	1.0	84.1
ccsA-ndhD_p5	ACCCAAGGCTTTGCTACTTC	CTCCCGGCCAACGTAATTTA	50.0	50.0	58.1	58.0	1491-1517 bp	1.0	83.9